Heater and thermocouple device for use in a test sensor and other assays, such as a diagnostic test sensor or strip, and a method of conducting an assay using a test sensor comprising the device. The heater and thermocouple device 100 comprising: a substrate 10; on the substrate, a first layer 12 of first conductive material of first conductivity comprising: a first thermocouple element 14, a first connector track 16 connected to the first thermocouple element, and a resistive heater element 20, at least part of the first thermocouple element is comprised of a portion of the heater element; and a second layer 22 of second conductive material of second conductivity comprising: a second thermocouple element 24 in contact with the first, a second connector track 26 connected to the second thermocouple element, and two heater connector tracks 36A, 36B spaced apart and connected to respective portions of the heater element, each on a respective side of the thermocouple elements.
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