The present disclosure relates generally to a method of separating ions according to their ion mobility, comprising (i) accumulating a first population of ions in a first region (12) of an ion mobility separator (10), (ii) separating said first population of ions according to their ion mobility in said first region (12) of said ion mobility separator (10), and (iii) accumulating a second population of ions in said first region (12) of said ion mobility separator (10) whilst said first population of ions are being separated according to their ion mobility in said ion mobility separator (10).
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