Identification of somatic origin or germline origin for acellular DNA

摘要

The present disclosure provides systems and methods for detecting somatic variants or germline variants from acellular DNA (cfdna).The above methodIs a step of receiving sequencing information from the subject CFCsThe sequencing information includes a cfdna sequencing system derived from the plurality of genomic lociDetermining the quantitative allele fraction (AF) value for each of the plurality of genomic loci based on the cfdna sequencingDetermining the standard deviation (STDev) for each of the AF values andProviding a STDev threshold and an AF threshold andDetermining whether STDev of each AF value exceeds or exceeds the STDev threshold, andIt may include determining whether each of the AF values exceeds or exceeds the AF threshold.