Cell culture observation method, cultured cell observation system, centrifugation system and cell passage system

摘要

PROBLEM TO BE SOLVED: To provide a method for facilitating or automating cell culture and observation by stabilizing and facilitating adjustment of focal length during cell observation. SOLUTION: An injection step of injecting cells contained in a culture solution into a cell culture observation container, a subculture step of subculturing the cells in the cell culture observation container, and a passage subcultured in the subculture step The cell culture observation container includes an observation step of observing cells, and a centrifugation step of separating cells subcultured in the subculture step into a culture solution and cultured cells by centrifugation. The method for observing cell culture is characterized in that the focal length is adjusted by using the mark with a microscope having an autofocus function in the observing step. [Selection diagram] Fig. 5