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SYSTEMS AND METHODS FOR MULTI-VIEW NONLINEAR OPTICAL IMAGING FOR IMPROVED SIGNAL-TO-NOISE RATIO AND RESOLUTION IN POINT SCANNING MULTI-PHOTON MICROSCOPY
SYSTEMS AND METHODS FOR MULTI-VIEW NONLINEAR OPTICAL IMAGING FOR IMPROVED SIGNAL-TO-NOISE RATIO AND RESOLUTION IN POINT SCANNING MULTI-PHOTON MICROSCOPY
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机译:点扫描多光子显微镜中改进的信噪比和分辨率的多视图非线性光学成像系统和方法
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摘要
Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.
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