Unique molecular identifier for single molecule sequencing and nucleic acid sequence characterization

摘要

Embodiments of the methods and/or systems (e.g., for improved single molecule sequencing, etc.) can prepare unique molecular identifier (UMI)-based molecular sets associated with a set of target nucleic acid sequences. step; A set of tagged nucleic acid molecules based on the set of UMI-based molecules, and a set of nucleic acid molecules tagged (e.g., including, etc.) corresponding to the set of target nucleic acid sequences to facilitate creation of a set of tagged nucleic acid molecules Step (eg, generating step); And/or facilitating single molecule sequencing with the tagged nucleic acid molecule (eg, performing, etc.).