PROCÉDÉS POUR L'ENRICHISSIMENT EN ACIDE NUCLEIC FÉTAL D'UN ÉCHANTILLON MATERNEL, BASÉ SUR LA MÉTHYLATION ET UTILISATION POUR LA QUANTIFICATION DE L'ACIDE NUCLEIQUE FÉTAL

摘要

Provided is a method for determining the amount of fetal nucleic acid in a sample comprising: a) contacting nucleic acid present in a sample from a pregnant female, which sample comprises differentially methylated fetal nucleic acid and maternal nucleic acid, with an agent that specifically binds methylated nucleotides; thereby producing bound nucleic acid; b) isolating the bound nucleic acid of (a), thereby enriching the fetal nucleic acid; and c) determining the amount of fetal nucleic acid by analyzing the amount of fetal nucleic acid of (b) at a plurality of loci selected from loci of SEQ ID NOs:1-89.