Method for recovering cultured cells from three-dimensional porous scaffolds

摘要

A method for efficiently recovering a large amount of cultured cells from a three-dimensional porous scaffold is provided. A culture process for culturing cells on a three-dimensional porous scaffold enclosed in a culture container, and an enzyme solution containing trypsin-EDTA and caseinase and / or collagenase is added to the culture container, Enzymatic treatment process of the cultured cells of the above, the pedestal moving circularly in the horizontal plane and the bottom surface of the culture vessel are in surface contact, and the vibration treatment is performed by applying vertical vibration to the cultured cells treated with the enzyme And a vibration processing step to be performed.