MODIFICATION DU GÉNOME D'ORGANITES À L'AIDE D'UNE ENDONUCLÉASE GUIDÉE PAR DES POLYNUCLÉOTIDES

摘要

Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.