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PROFILAGE PHÉNOTYPIQUE DE CELLULES TUMORALES CIRCULANTES D'UN CARCINOME HÉPATOCELLULAIRE POUR LA SÉLECTION D'UN TRAITEMENT
PROFILAGE PHÉNOTYPIQUE DE CELLULES TUMORALES CIRCULANTES D'UN CARCINOME HÉPATOCELLULAIRE POUR LA SÉLECTION D'UN TRAITEMENT
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摘要
Methods and kits for detecting hepatocellular carcinoma recurrence or metastasis, and of measuring markers of hepatocellular carcinoma, including markers of hepatocellular carcinoma recurrence or metastasis, in a blood sample obtained from a subject by (a) isolating circulating tumor cells (CTCs) by contacting a blood sample obtained from the subject with a set of capture antibodies, wherein the capture antibodies specifically bind asialoglycoprotein receptor (ASGPR), Glypican-3, and epithelial cell adhesion molecule (EpCAM); (b) contacting the isolated CTCs with an antibody that specifically binds vimentin; and (c) measuring the number of vimentin-positive CTC.
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