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一种用于检测血清中PF4含量的抗体对及其用途

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摘要

本申请提供一种用于检测血清中PF4含量的抗体对及其用途，包括与PF4的N端表位即SEQ ID NO:2所示氨基酸序列相结合的单克隆抗体AntiPF4_N和与PF4的C端表位即SEQ ID NO:3所示氨基酸序列相结合的单克隆抗体AntiPF4_C，所述单克隆抗体AntiPF4_N包括SEQ ID NO:7所示氨基酸序列的轻链可变区和SEQ ID NO:11所示氨基酸序列的重链可变区，所述AntiPF4_C包括SEQ ID NO:17所示氨基酸序列的轻链可变区和SEQ ID NO:21所示氨基酸序列的重链可变区。本申请将PF4抗原分成N端(SEQ ID NO:2)和C端(SEQ ID NO:3)两段序列，并用这两段序列分别免疫小鼠，筛选到高特异性和高灵敏性的抗体对AntiPF4_N和AntiPF4_C，通过该抗体对可以有效的测定肝素使用后血清中PF4的含量，进而便于指导肝素的使用。

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公开/公告号CN111793131A

专利类型发明专利
公开/公告日2020-10-20

原文格式PDF
申请/专利权人廊坊天光生物技术有限公司;
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申请/专利号CN202010914313.3
发明设计人刘静;刘继来;程勇;王征;
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申请日2020-09-03
分类号C07K16/24(20060101);G01N33/68(20060101);C12N15/13(20060101);
代理机构11435 北京志霖恒远知识产权代理事务所(普通合伙);
代理人韩亚伟
地址 065500 河北省廊坊市固安县新兴产业示范园区肽谷生物医药产业园孵化楼
入库时间 2023-06-19 07:58:45

法律信息

法律状态公告日

法律状态信息

法律状态
2022-01-14

发明专利申请公布后的撤回 IPC(主分类):C07K16/24 专利申请号:2020109143133 申请公布日:20201020

发明专利申请公布后的撤回

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1. 一种用于检测血清中PF4含量的抗体对及其用途 [P] . 中国专利： CN113150142A . 2021-07-23
2. 一种用于检测血清中RANKL含量的抗体对及其用途 [P] . 中国专利： CN113150158A . 2021-07-23
3. Isolated negative-sense single stranded rna virus, isolated or recombinant nucleic acid or its specific functional mpv fragment, vector, host cell, isolated or recombinant proteinaceous molecule or its specific functional mpv fragment, antigen, antibody, method for identifying a viral isolate as a mpv, viral isolate, methods for virologically and serologically diagnosing a mammalian mpv infection, diagnostic kit for diagnosing a mpv infection, uses of a virus, a nucleic acid, a vector, a cell host, a proteinaceous molecule or fragment thereof, an antigen, or an antibody, pharmaceutical composition, methods for treating or preventing an mpv infection and respiratory disease, and for obtaining an antiviral agent useful in the treatment of disease. of the respiratory tract, antiviral agent, use of a viral agent, methods for virologically diagnosing and serum logically a bird apv infection, use of a diagnostic test, and method for detecting an antibody directed against mpv in a sample [P] . BR0206591A . 2005-02-01

机译：分离的负义单链RNA病毒，分离的或重组的核酸或其特定功能的mpv片段，载体，宿主细胞，分离的或重组的蛋白质分子或其特定的功能mpv片段，抗原，抗体，将病毒分离物鉴定为mpv，病毒分离物，用于病毒学和血清学诊断哺乳动物mpv感染的方法，用于诊断mpv感染的诊断试剂盒，病毒，核酸，载体，细胞宿主，蛋白质分子或其片段，抗原的用途，或抗体，药物组合物，用于治疗或预防mpv感染和呼吸道疾病以及获得可用于治疗疾病的抗病毒剂的方法。呼吸道，抗病毒药，病毒药的使用，禽apv感染的病毒学诊断和逻辑诊断方法，诊断测试的使用以及检测样品中针对mpv的抗体的方法
4. Hmw protein of the isolated chlamydia species, isolated nucleic acid molecule, recombinant expression vector, transformed host cell, isolated recombinant protein or fragment or analog thereof, immunogenic composition, antigenic composition, processes to produce an immune response in an animal, anti- serum, antibodies, diagnostic reagent, to detect anti-chlamydia antibodies in a test sample, to detect the presence of chlamydia in a test sample, to prevent, treat or ameliorate a chlamydia-related disorder in a host in need of this treatment , and to determine the presence of nucleic acid encoding an hmw protein or a fragment or analog thereof in a sample, diagnostic kits to detect antibodies to chlamydia, and to determine the presence of nucleic acid encoding an hmw protein or fragment or analog thereof in a sample, vaccine composition, and pharmaceutical composition The [P] . BR9813841A . 2000-10-03

机译：分离的衣原体物种的Hmw蛋白，分离的核酸分子，重组表达载体，转化的宿主细胞，分离的重组蛋白或其片段或类似物，免疫原性组合物，抗原性组合物，在动物中产生免疫应答的过程，抗血清，抗体，诊断试剂，用于检测测试样品中的衣原体抗体，检测测试样品中的衣原体，预防，治疗或缓解需要此治疗的宿主中的衣原体相关疾病，并确定样品中是否存在编码hmw蛋白或其片段或类似物的核酸，诊断试剂盒以检测针对衣原体的抗体以及确定样品，疫苗组合物中是否存在编码hmw蛋白或其片段或类似物的核酸和药物成分
5. pencicillin-binding protein, nucleic acid, antibody or antibody fragment, medicament, pharmaceutical composition, uses of at least one penicillin-binding protein or fragment or variant or fragment thereof, of at least one nucleic acid and of at least one antibody or antibody fragment, in vitro antibody detection methods directed against neisseria meningitidis infection from a penicillin-binding protein, nucleic acid, antibody or an antibody fragment, pharmaceutical composition of at least one penicillin-binding protein or fragment or variant or variant thereof, of at least one nucleic acid and at least one antibody directed against neisseria meningitidis infection from a biological sample of a mammal and in vitro diagnosis of neisseria miningitidis infection from a biological sample from a mammal and monoclonal antibody [P] . BRPI0610802A2 . 2016-11-16

机译：青霉素结合蛋白，核酸，抗体或抗体片段，药物，药物组合物，至少一种青霉素结合蛋白或其片段或变异体或片段，至少一种核酸和至少一种抗体或抗体片段的用途，针对脑膜炎奈瑟菌的青霉素结合蛋白，核酸，抗体或抗体片段感染的体外抗体检测方法，至少一种青霉素结合蛋白或其片段或变异体或变异体，至少一种核酸的药物组合物酸和至少一种针对哺乳动物生物样品中脑膜炎奈瑟氏菌感染的抗体以及来自哺乳动物生物样品中的矿业性奈瑟氏球菌感染的体外诊断和单克隆抗体