ラット股関節 nerve growth factor 投与モデルにおける炎症性サイトカイン及び疼痛行動評価

摘要

[SUMMARY] 【Objectives】 To determine gait and inflammatory response in a rat hip pain model produced by intra-articular injection of nerve growth factor（NGF）. 【Methods】 Using 36 8-week-old male Sprague Dawley rats, 30 μl of saline（sham-operated group; n＝12）, 30 μL of 50 μg/mL of NGF（NGF50 group; n＝12）, and 30 μL of 100 μg/mL of NGF（NGF100 group; n＝12）were injected into the left hip. The gait of 6 rats in each group was analyzed using a CatWalk system. The levels of inflammatory cytokines were determined with the synovium and femoral head. 【Results】 The maximum contact area was significantly decreased in the NGF100 group compared with in the sham-operated group on day 14. Swing speed significantly decreased in the NGF50 and NGF100 groups compared with in the sham-operated group on day 14. Duty cycle significantly decreased in the NGF50 and NGF100 groups compared with the sham-operated group on day 14. TNF-α, IL-1β, and IL-6 were significantly elevated in NGF50 and NGF100 groups compared with the sham-operated group. 【Conclusions】 NGF into the rat hip joint induces an increase of inflammatory cytokines in the synovium and elicits pain escape behavior.