Study on the prognostic value of p53 and PTEN immunomarkers for endometrial cancer using immunohistochemistry

摘要

Introduction: Mutation in p53 and phosphatase and tensin homolog(PTEN) genes are reported to be prevalent in endometrial cancer. The presentstudy aimed to evaluate the immunohistochemical expression of p53 and PTENproteins in endometrial cancer among women with hysterectomy.Methods: Inthis cross-sectional study, 40 paraffin-embedded endometrial cancer sampleswere collected during 2015 to 2016, from women with hysterectomy in Al ZahraHospital, Tabriz, Iran. The histopathological observation was performed toconfirm endometrial cancer and its grade. Immunohistochemistry (IHC) was donefor p53 and PTEN biomarkers. Data were analyzed by SPSS.Results: Thirty-three(82.5%), six (15.0%) and one (2.5%) out of 40 samples were endometrioidendometrial adenocarcinoma, serous carcinoma and clear cell adenocarcinoma,respectively. Furthermore, 5, 16 and 19 out of 40 studied samples belonged tograde I, II and III, respectively. The IHC observation showed that p53expression in 9 (22.5%) was positive, while the rest 31 (77.5%) samples werep53 negative. Moreover, PTEN expression was observed in 10 (25%) samples and 30(75.0%) samples were PTEN negative. The sensitivity of p53 and PTEN fordiagnosis of endometrial cancer was calculated as 56.3% and 80%, respectively.Conclusion:The IHC markers, p53 and PTEN, show heterogeneousresults as diagnostic and prognostic markers for endometrial carcinoma and aresuggested to be used along with other markers for such purposes.