Influence of Particulates on Occupational Lung Disease

摘要

The ability of alveolar macrophages (AM) to phagocytize uncoated andbenzo(a)pyrene (50328) (BaP) coated particulates was investigated. AM from male Syrian-golden-hamsters and Sprague-Dawley-rats were used. Particulates included crystalline-silica (14808607); precipitated, gelled, and fumed amorphous-silica (7631869); ferric-oxide (1309371); and aluminum-oxide (1344281). All forms of amorphous silica had smaller particle sizes and were cytotoxic, suggesting that more of the dose for the amorphous silicas having more silanol groups being more cytotoxic. These same properties were important in the metabolic activation of BaP by AM. At a similar dose of BaP and particle, all of the BaP coated particles produced more BaP dihydrodiols than BaP alone. AM exposed to particles coated with BaP responded differently than either BaP or particles alone at noncytotoxic doses, For metabolism, DNA binding and cytotoxicity parameters, the surface area of the particle appeared to play a major role.