Modulation of Lymphocyte Locomotion through Type 1 Collagen by Anti-beta(sub 1) Integrin Antibodies

摘要

We investigated the role of collagen-binding VLA integrins in lymphocyte locomotion using selected antibodies specific to distinct B, epitopes and two (different) alpha chains in an attempt to disrupt lymphocyte interaction with the collagen matrix and thereby alter the net movement of lymphocytes through the matrix. Results indicate that antibody 33B6 to the beta(sub 1) 1.2 epitope, when simultaneously introduced to the cells with collagen substrate, could inhibit lymphocyte locomotion of both PBMC and donor-matched anti-CD3 / IL-2 activated T cells (aT) into type 1 collagen. Antibody TS2-7 to the alpha(sub 1) chain of VLA-1 stimulated locomotion of both PBMC and aT. We hypothesize that these antibodies modulate the interaction of integrin receptor with collagen substrate thus altering locomotory behavior through collagen in vitro.