STUDY OF THE INCORPORATION OF URIDINE 3H IN NEURONS BY VARIOUS AUTORADIOGRAPHIC METHODS

摘要

A study was made of the incorporation of undine-H in the RNA's in the nerve cells with recently developed high-resolution autoradio-graphic processesnThe cerebral hemispheres of 14-day-old chick embryos were removed, cut in pieces, and incubated in vitro for 30 and 60 mm in the presence of uridine H (10, 50 µCi/ml) The pieces were then fixed and prepared for autoradiographynThe sections were covered with a layer of nuclear emulsion (Gevaert Nuc 7 15 or Ilford K5) After drying- by temperature gradient, the plates were exposed for 1 to 4 days as required After activation with gold, the autoradiographs were developed in amidol or ferrous oxalate for suitable periods giving the best signal/noise ratio.nOur methods revealed cytoplasmic radioactivity after only 30 min incubation, after one hour the activity became relatively strong.