Molybdenum--Substrate Interactions in Nitrogenase: An EXAFS Study

摘要

The sensitivity of x-ray absorption edge spectra and extended x-ray absorption fine structure (EXAFS) to the environment of metal atoms make x-ray absorption spectroscopy a useful probe of the environment of the molybdenum in nitrogenase. It is of particular interest to investigate any perturbations of the environment of the molybdenum due to the presence of nitrogenase substrates. The results of measurements of the x-ray absorption spectra of nitrogenase samples by the absorption and fluorescence detection techniques are compared. The procedures used for the growth of Azotobacter vinelandii for the production of nitrogenase and the procedures used for the purification of nitrogenase are described in detail. (ERA citation 04:001432)