Fundamental Studies in Continuous Annular Chromatography

摘要

The effects on separation performance of bed-rotation rate, bed loading, and feed concentrations were investigated using a pressurized continuous chromatograph. Ion-exchange chromatography was used to separate (Ni(NH sub 3 )/sub 6-x/(H sub 2 O)/sub x/) exp 2+ , (Co(NH sub 3 ) sub 4 CO sub 3 ) exp + , and (Co(NH sub 3 ) sub 5 CO sub 3 ) exp + with 1.6 M (NH sub 4 ) sub 2 CO sub 3 of pH 9.0 as eluant. The experimental results were compared with predictions of plate theory and with a theoretical multicomponent chromatography model developed by Rhee. Experimentally observed band positions are accurately predicted by both models in the low feed concentration region but only by the Rhee model in the high concentration region. The experimentally determined bandwidths, however, are underestimated by both models, but more severely for the cobalt complexes by the Rhee model which does not allow dispersion. A correction to the experimental bandwidths was necessary to account for the discrete nature of the sampling. (ERA citation 06:035240)