Effect of Diamide on Potassium Transport and Cellular Morphology in Mouse L-Cells

摘要

The effects of diamide (diazenedicarboxylic acid bis (N,N'-dimethylamide)) on the transport of potassium in mouse L-cells have been investigated using exp 86 Rb exp + as a tracer. Active, ouabain-sensitive uptake is reduced after 0.4 to 0.6 mol/L diamide treatment. The size of reduction depends on the temperature and the presence of glucose in the medium. These results suggest that the elimination of reduced glutathione by diamide is the major factor controlling the level of K exp + transport in treated L-cells. In addition to decreasing active transport, diamide produces dramatic changes in cellular ultrastructure, probably through altered Na exp + /K exp + balance and its action on tubulin. Clear organelle-free regions appear surrounded by vacuoles and swollen mitachondria regions. The clear areas of cytoplasm eventually pinch off from the cell. (Atomindex citation 14:731662)