Interaction Between Chemotherapeutic Agents and Biological Processes in Isolated Mammalian Cells. Coordinated Programme on Improvement in Radiotherapy of Cancer Using Modifiers of Radiosensitivity of Cells. Final Report for the Pe

摘要

In the investigations presented in this report the intention was to examine how peptichemio (PTC-alkylating cytostatic agent) acts either alone or in combination with hyperthermia and X-irradiation on the biological processes in isolated mammalian cells. For controlling this action several parameters have been used: the estimation of cell survival, detection of single strand breaks (SSB) in DNA and chromosomal aberrations analysis. The obtained results show that the survival of L cells exposed to PTC is dose and time dependent. Hyperthermia at 42 exp 0 C potentiates killing effect of PTC treated cells. Maximal effect is obtained under simultaneous treatment. Simultaneous exposure of cells to PTC and hyperthermia before X-irradiation results, depending on drug concentrations, in a reduction or elimination of the shoulder and increase the slope of the survival curves. When cells were simultaneously exposed to PTC and hyperthermia the induction of SSB in DNA molecule is observed. The repair of SSB depends on the amount of SSB induced and on the post-incubation temperature. PTC applied after X-irradiation suppresses the repair of SSB induced by X-irradiation, depending on exposure time and drug's concentration. The incidence of chromosomal aberrations in cells exposed to PTC or Adriamycin (ADR) depends on concentration and exposure time. Simultaneous exposure of cells to hyperthermia (42 exp 0 C. (Atomindex citation 14:727849)