Evaluation to Determine Potential Hazards of Dermal Contact with Sh-82-009,2,2'-dithio-bis-benzothiazole with Cover Letter dated 092084 Sequence C.

摘要

The ability of mercaptobenzothiazole to induce specific locus mutation at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S9 metabolic activation. Based on preliminary toxicity determinations, nonactivated and S9 activated cultures were evaluated at concentrations up to 150ug/ml and 120ug/ml, respectively, producing a wide range of toxicities. Nonactivated cultures at the highest toxicities (less than 10% relative growth) produced 1.8- to 8.7-fold increases in the mutant frequency relative to the solvent (DMSO) and untreated controls. With S9 activation, treatments producing 7 - 20% relative growth increased the mutant frequency 1.7- to 2.7-fold compared to the solvent and untreated controls. Mutagenicity Evaluation of Mercaptobenzothiazole in the Mouse Lymphoma Forward Mutation Assay with Cover Letter dated 011885 & EPA Acknowledgement Sequence E.