Exploratory Research on Mutagenic Activity of Coal-Related Materials Using Statistical Evaluation.

摘要

Potential synergism among components of fuel-derived mixtures is being investigated by comparison of mutagenic activities of ''reconstructed wholes'' with the sum of mutagenic activities of individual fractions. Results of forward mutation assays of samples of Lummus Product and Lummus Feed contrasted with those obtained from reverse mutation assays. Data from assay of both materials gave indications of the likelihood of interactions among fractions contributing to increased mutagenicity. The ability of fuel sample fractions to influence biological activities of individual components is also being studied. In these mutagenicity assays 2-aminoanthracene (2AA), as a model aromatic amine, is tested in the presence of the hexane fraction of PDU-9. Data from these assays show an enhanced mutagenic response as a result of mixing these two materials. This enhancement is not solely due to bacteriotoxic effects. It is, however, probably related to metabolism of the 2AA and/or hexane fraction. The PDU-9 hexane fraction (organic solvent extract) is presently being analyzed chemically using HPLC and fluorescence techniques. Various fractions are being collected so as to identify and characterize the major peaks. These fractions will also be analyzed for their mutagenicities using the forward mutation assay. 5 figs. (ERA citation 11:047210)