Enzymological Studies of One-Carbon Reactions in the Pathway of Acetate Utilization by Methanogenic Bacteria: Progress Report, June 1, 1987-May 31, 1989.

摘要

The following major advances were made in the past two years: both acetate activating enzymes were characterized and the regulation of synthesis was studied; direct biochemical evidence for the proposed function of the CODH was obtained, and the CODH complex was further characterized; an electron transport chain was reconstituted with CODH, ferredoxin, and membrane components that catalyzed oxidation of carbon monoxide and evolution of hydrogen; the ferredoxin was characterized; five new enzyme activities were discovered with potential for involvement in growth and/or methanogenesis from acetate; probes were developed for isolation of the genes encoding the principle enzymes of the pathway. 6 refs., 3 figs.