Drug-DNA interactions studied by x-ray crystallography. (Progress report).

摘要

The broad objectives of this research proposal are to understand how two different intercalators --- actinomycin D and ethidium -- bind to DNA. Both drugs are widely used in the molecular biology area, and providing a detailed understanding of their stereochemical interactions with DNA could lead to valuable new insights into DNA structure and function. With this in mind, we have crystallized two different complexed that contain these drug molecules complexed to DNA-like oligomers and are carrying out their crystallographic analyses. The specific aims of this research proposal are to solve and to refine these structures to the highest possible resolution. This will involve recollecting the intensity data using the synchrotron radiation source on larger crystals of both complexes that are now available. 65 refs., 12 figs.