X-ray induction of micronuclei in human lymphocyte subpopulations differentiated by immunoperoxidase staining.

摘要

In this report we sought to confirm the radiosensitivity of human peripheral blood lymphocyte subpopulations using a micronucleus assay. Mononucleated cells isolated from peripheral blood were irradiated with X rays. After being cultured for 3 days, cells were fixed and stained using the immunoperoxidase staining technique. Lymphocyte subpopulations were characterized by means of the monoclonal antibodies Leu4 (CD3), Leu2a (CD8), and Leu19 (CD56). Dose-response curves were obtained by scoring the number of micronuclei in binucleated cells that reacted with a specific antibody and were then stained. The dose response of CD8(sup +) (suppressor/cytotoxic) cells was quite similar to that of CD3(sup +) (pan T) cells. In comparison, CD56(sup +) (natural killer) cells were significantly less sensitive, although scorable binucleated CD56(sup +) cells made up less than 4 % of the total number of binucleated cells. (author). (ERA citation 18:037383)