Antibody Response of Monkeys to Invasion Plasmid Antigen D after Infection withShigella spp

摘要

The antigen preparation most often used for measuring antibodies to virulenceassociated proteins of Shigella spp. consists of a mixture of proteins (including IpaB, IpaC, IpaD, and VirG*) extracted from virulent shigellae with water. To overcome the lack of specificity for individual antigens in the water-extract ELISA, the ipaD gene from S. flexneri has been cloned, expressed to high level, and purified for use in a new ELISA for the measurement of antibodies against IpaD in monkeys and humans challenged with shigellae. The IpaD ELISA for serum IgG and IgA correlated well with the water extract ELISA in that monkeys infected with S. flexneri or S. sonnei responded with high serum antibody titers in both assays. The IpaD assay required less antigen per well, had much lower background levels, and did not require 'correction' with antigens from an avirulent organism. In conjunction with the water-extract ELISA, it was possible to identify infected animals that did not respond against IpaD but did produce antibodies that reacted in the water-extract ELISA. This indicates that even though IpaB, IpaC and IpaD are essential for the invasiveness phenotype, the infected host does not always produce antibodies against all components of the invasiveness apparatus.