Detect antibody of enolase using mimical antigen for diagnosis of invasive Candadiasis

摘要

The diagnosis of invasive candidiasis (IC) was attempted by detection of circulating antibody against enolase by an ELISA assay established using the mimical antigen selected from phage display, and this method was compared with the conventional culture method. One hundred and thirty sera from patients with various Candida infection confirmed by microbiological culture were collected. The surrogate peptides were used for coating antigen to detect antibodies against enolase in the patients' sera, and then the method was evaluated for distinguishing between invasive and superficial infection of C. albicans, and the cross reaction with other Candida infection. The antibody was detectable in all of 35 patients with invasive infection of C. albicans, 2 out of 42(4. 8%) patients with superficial infection of C. albicans and 3 out of 18 (16. 7%) patients with invasive infection of C. tropicalis. The enolase antibody was negative for sera from patients infected with C. glabrata, C. parapsilosis and un-identified Candida, no matter invasive or superficial.