Purification and Analysis of a Recombinant Human Anti-Cholera Toxin B Antibody

摘要

A large semi-synthetic recombinant antibody library was constructed based on ahuman anti-tetanus library. The library was used to isolate a human anti-cholera toxin B antibody cidne, which is expressed in XL-1 Blue E. Coli bacteria. The clone, B-27 cholera Fab, was expressed in small scale and purified by a two column procedure, which uses the histidine affinity tag, which was engineered into the protein. The purified cholera Fab was tested by ELISA to confirm specificity and by surface plasmon resonance to determine affinity binding constants.