Compatibility of CW Agent Degrading Enzymes with Disinfectants and Foams

摘要

Over the years our group developed a non-corrosive, non-toxic, environmentally safe and userfriendly chemical agent decontaminant (advanced catalytic enzyme system - ACES) in a dry powder form. The ACES is capable of hydrolyzing/detoxifying organophosphorus (OP)-based neurotoxic chemical warfare (CW) agents, i.e. G-type, V-type, and related OP based hazardous industrial materials. Bacterial enzymes, OPH (V- and G-type and pesticides), OPAA (G-type) and squid DFPase (G-type) are the three key biocatalysts capable of detoxifying toxic materials. Retention of catalytic activity of ACES in the presence of several water-soluble and biodegradable commercial fire-fighting agents/foams, degreasers, and laundry detergent, provides effective and efficient decontamination not just for large areas, but also for fire suppressing. In addition, the formulation affords personnel decontamination in skin cream/lotions, and personnel protection through enzyme-impregnated clothing and filters. Here, we report the retention of catalytic activity of ACES in commercial disinfectants (Biocidal ZF and EcoTru ). Over 50% enzyme activity was retained in the presence of Biocidal ZF . Whereas a 40% enzyme activity was detected in the presence of EcoTru , over 65-85% enzyme activity was retained when enzymes were protected using dendritic polymers. Biological decontamination experiments with biological warfare agent (BWA) surrogates showed varying degrees of bactericidal activity (2-10 log kill) when these disinfectants and several commerical foams were tested individually. These commercial compounds were compatible with the ACES enzyme activity. Our findings are consistent with the concept that ACES can be incorporated into commercial disinfectants with a real potential for developing broad range decontamination systems for CBW agents.