Determining Extracellular Matrix Deposition Quantity and Composition from Cells in Response to Electronic Materials

摘要

The project objectives were to determine the composition and quantity of the extracellular matrix (ECM) that is deposited by the cells when placed in contact, either in vivo or in vitro, with electronic materials. In addition, a defined serum-free in vitro system developed in this laboratory was used to identify, favorable and unfavorable materials, both biological and non- biological, that could be used to enhance interactions of cells and tissue with electronic components. Pure cultures of embryonic hippocampal neurons do not produce large areas of ECM spread over the coverslip. The cells deposit ECM proteins as a barrier to the inorganic DETA surface, possibly as a way to control their microenvironment, especially since the protein composition did not differ as a function of surface hydrophilicity or hydrophobicity. The neurons secrete laminin and collagen, but it is mostly concentrated around the soma and under the processes. In pure in vitro culture, the neurons do not secrete fibronectin, which seems to be the role of glial cells.