Dietary Influences on Alpha-Methylacyl-CoA Racemase (AMACR) Expression in the Prostate; Annual rept. 1 Apr 2007-30 Mar 2008

摘要

daAlpha Methyl Acyl CoA Racemase (AMACR) a peroxisomal and mitochondrial enzyme is up regulated in majority of prostate cancers (PCa). This enzyme is involved in the breakdown of phytanic & pristanic acids which are derived primarily through the ingestion of dairy and red meat products. There are no studies done so far that have examined the relationship of this enzyme with red meat and dairy intake and therefore PCa risk. The current research focuses on examining the relationships between AMACR expression in the prostate and phytanicipristanic acid levels in the blood and prostate. So far 19 patients with Pca have been recruited in the study. Research staff has been trained to obtain dietary information as well as process tissue and blood samples from participants. Protocols for processing biological samples have been established. Preliminary optimization of laboratory assays including assays for quantifying AMACR using automated image analysis tools have been developed.