The Manufacture and Study of Stroma Free Hemoglobin Solution

摘要

Primates exchange transfused to zero hematocrit with a modified red cell hemolysate maintain normal oxygen consumption. Their hemoglobin mass was reduced to 6 grams %, and the P50 was decreased to 14 mm Hg. Oxygen consumption was maintained by increasing the extraction ratio, with no change in cardiac output. This was surprising to us since the typical response to hemodilution is an increase in cardiac output. Some form of hypoxic heart failure could have been responsible or these animals might not have had sufficient stimulus to increase their cardiac output. The purpose of the current study was to evaluate the ability of such animals to increase their cardiac output in response to beta-adrenergic stimulation. This was accomplished by using a standardized bolus infusion of isoproterenol. It has been shown that the hypoxic and ischemic myocardium has an altered response to this drug.