Delivery Systems for Bone Morphogenetic Protein for Repair of Battle and Civilian Incurred Bone Injuries

摘要

Research on bone regeneration under the influence of the bone morphogenetic protein (BMP) aggregates and isolated protein is discussed. The bone morphogeneic protein (BMP) is prepared from bovine or human cortical bone. The response to BMP is assayed in vivo by measuring the biosynthetic products of induced differentiation of perivascular connective tissue of muscle into chondro-osteoprogenitor cells by the quantity of cartilage-type cell biosynthetic products of connective tissue cell outgrowths of neonatal rat muscle. UCLA is contracted to test BMP induced bone formation in unstable spinal columns and in 4 cm long bone segmental defects in dogs simulating missing bone substance from battle incurred high velocity bullet or scrapnel wounds in military personnel. The defects will be implanted with BMP without and with iliac crest cancellous bone marrow. The experimental plan includes tests of synergistic effects of various recently discovered growth factors on the response to BMP. The batches of BMP implanted in dogs will be sent to USA RCD, USA IDR group for research on biodegradable non-immunogenic delivery systems. The USA RCD, USA IDR group will test BMP in delivery systems for craniofacial defects while UCLA will test the same systems on long bone and fusions of unstable spinal columns. The program is based on the hypothesis that BMP is a cell differentiation factor or morphogen as distinguished from a growth factor or mitogen.