Viral Oncolytic Therapeutics for Neoplastic Meningitis, July 2013 (Principal Investigator, Kuruppu).

摘要

Neoplastic meningitis is a fatal complication of breast cancer for which there is no curative treatment at present. The project aims to develop a novel, safe and efficient therapy for neoplastic meningitis HSV-1 oncolysis therapy. In the first year, after completing the stated tasks for that year, we developed stable breast cancer cell lines that express the renilla luciferase with the aim of using them to non-invasively image their tumors with bioluminescence. While waiting for the animal facilities at MGH to be set up in order to complete the remainder of the studies stated for the second year, we commenced developing a mouse model of meningeal metastases selecting the tripe negative human breast cancer cell line MDA-231 which we had created. During the second year we have developed a mouse model of meningeal metastases in mice and characterized the progression of meningeal metastases (Neoplastic meningitis) over time with sequential imaging with bioluminescence and MRI. The images were correlated with in vitro staining of brain sections with H&E and the development of tumors compares with the disease progression in humans. The fully characterized model was presented at the annual meeting of the Society of nuclear medicine and molecular imaging (SNMMI) in Vancouver (June, 2013). This model is being written up for publication in Cancer Research. In addition, studies of viral and cellular kinetics with bioluminescence and PET are being written up for publication.