Vector Competence of Mosquitoes for Arboviruses

摘要

Infection rates of Aedes aegyptus to dengue 2 viral infection following feedingon artificial bloodmeals were improved by modifying the composition of the artificial bloodmeals. Analysis of these results was improved by reducing the days of extrinsic incubation from 11 to 9, thus avoiding the significant mortality (often 75%) which occurred between days 9 and 11 and minimizing variability from experiment to experiment. A monoclonal antibody has been isolated and used to purify WS2 and WS2 antigens, which are specific to virus susceptible and virus refractory strains of Culex tarsalis, respectively. It was suggested that the nascent WR2 antigen is processed to the WS2 form through posttranslational modification. Hybridomas from mice immunized with WR2 antigen were produced, however, no hybridoma subclones producing antibody were detected. Clones of either of two cell lines may provide a cell culture model for alphaviral titer modulation.