Synthesis of Chemiluminescent Flavoabzymes and Their Use in Metal Ion Detection

摘要

The ultimate goal of this research is to develop chemiluminescent (CL) abzymesand a technology for their use in metal ion detection. Four objectives must be reached in sequential order: lst, a molecule (hapten) which resembles in space requirements an intermediate in a CL reaction must be synthesized; 2nd, a hapten protein conjugate (antigen) must be prepared and used to obtain monoclonal antibodies (Mab's); 3rd, one or more of the Mab's must catalyze the given CL reaction; and 4th, the hapten must be modifiable such that the CL Mab's become specific complexers of metal ions. Accomplishment of the 1st objective would provide the first Mab mimic of bioluminescence. Attempts to synthesize a hapten to generate Cl flavoabzymes will be discussed as will progress in the synthesis of a hapten to be used to prepare abzymes to catalyze the CL reaction of H202 with oxalate esters. Monoclonal antibodies (Mab's), Abzymes, hapten, Antigen, Chemiluminescence (CL), Bioluminescence.