Structural and Biophysical Characterization of a Glutamate Receptor.

摘要

The immediate objectives of this work, namely to isolate, purify, structurally characterize and clone insect muscle GLuR were not realised, mainly because of unforseen technical difficulties, some of which have yet to be overcome. However, unexpectedly rapid developments in the molecular for this class of receptor have been made. We have taken advantage of these developments by designing new molecular biological procedures for over-expressing cloned mammalian GluR subunits. Since the long-term aim of this project is to produce biosensors based upon GluR, the availability of pure mammalian GluR is clearly advantageous in this respect.