Insecticide resistance in Frankliniella occidentalis: Corroboration of laboratory assays with field data and cross-resistance in a cypermethrin-resistant strain

摘要

Field efficacy of five insecticides to a greenhouse strain of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) of known resistance status was studied. In a greenhouse trial, the mortality of a F. occidentalis strain (Uncali) which exhibited resistance only against cypermethrin in laboratory assays, reached 56%, 68%, 82%, 94% and 99%, 24 h after application of abamectin, cypermethrin, endosulfan, malathion and methomyl, respectively. In the laboratory, for larval mortality equal to that achieved in the field, much lower doses - half of the field dose of cypermethrin and endosulfan, and one-third and one-sixth of malathion and methomyl, respectively, were required in topical application. Because of differences between laboratory and field responses of F occidentalis to insecticides, a sound resistance evaluation program to determine field efficacy of insecticides is needed. Resistance increased 28.0- and 139.3-fold at LC50 in the F. occidentalis strain subjected to 27 and 44 cycles of cypermethrin selection, respectively. A slight cross-resistance of two- to threefold was found with endosulfan, malathion and methomyl and no cross-resistance was observed with abamectin.