Micropropagation of Aloe vera L. and estimation of potentially active secondary constituents.

摘要

To achieve micropropagation of Aloe vera, adventitious shoot buds were cultured on Murashige and Skoog's (MS) (1962) media containing different combination and concentrations of auxins and cytokinins. Out of the various combinations tried shoot establishment was achieved on MS+BAP (1 mgl-1)+Kn(1 mgl-1) and MS+BAP(1 mgl-1)+NAA(5 mgl-1); and was faster in former medium as compared with the latter. Multiplication rate was achieved maximum (16.8 per cent) when MS medium was supplied with BAP (2.5 mgl-1). Thus, shoot multiplication rate of 302.5 and 288 was reported when shoots established on MS+BAP(1 mgl-1)+Kn(1 mgl-1) and MS+BAP(1 mgl-1)+NAA (5 mgl-1) respectively were multiplied on MS+BAP(2.5 mgl-1); though mean leaf number and leaf length were more in the latter. Hundred per cent and faster root formation occurred on MS basal medium and plantlets were hardened on moist cotton in hardening trays. The sterol content in micropropagated plants increased significantly in tip, middle and basal regions of leaves. The quantity of phenols was recorded maximum in middle portion of leaves of natural as well as tissue cultured clones but content was more in latter. By tissue culture technique Aloe plants could be propagated at much faster rate and with more potentially active secondary constituents.