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DNA barcodes for biosecurity: invasive species identification

机译:用于生物安全性的DNA条形码:入侵物种识别

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摘要

Biosecurity encompasses protecting against any risk through 'biological harm', not least being the economic impact from the spread of pest insects. Molecular diagnostic tools provide valuable support for the rapid and accurate identification of morphologically indistinct alien species. However, these tools currently lack standardization. They are not conducive to adaptation by multiple sectors or countries, or to coping with changing pest priorities. The data presented here identifies DNA barcodes as a very promising opportunity to address this. DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach. Species identifications were compared with the historical dataset obtained by PCR-RFLP of nuclear rDNA. There was 90 and 96% agreement between the methods for these species, respectively. Improvements included previous tussock moth 'unknowns' being placed to family, genera or species and further resolution within fruit fly species complexes. The analyses highlight several advantages of DNA barcodes, especially their adaptability and predictive value. This approach is a realistic platform on which to build a much more flexible system, with the potential to be adopted globally for the rapid and accurate identification of invasive alien species.
机译:生物安全包括通过“生物危害”防范任何风险,尤其是有害生物昆虫传播产生的经济影响。分子诊断工具为快速,准确地识别形态上不清楚的外来物种提供了宝贵的支持。但是,这些工具当前缺乏标准化。它们不利于多个部门或国家的适应或不利于应对不断变化的有害生物重点。此处提供的数据将DNA条形码识别为解决此问题的极有希望的机会。使用cox1序列条形码方法重新分析了过去十年在新西兰边境截获的tus蛾和果蝇标本的DNA。将物种鉴定与通过核rDNA的PCR-RFLP获得的历史数据集进行比较。这些物种的方法之间分别有90%和96%的一致性。改进措施包括将以前的tus蛾“未知”放入科,属或种,并在果蝇种复合物中进一步解决。分析突出显示了DNA条码的几个优点,尤其是它们的适应性和预测价值。这种方法是一个现实的平台,可以在此平台上构建更加灵活的系统,并有可能在全球范围内迅速而准确地识别外来入侵物种。

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