Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid,Myzus persicae(Hemiptera:Aphididae)and the rice brown planthopper,Nilaparvata lugens(Hemiptera:Delphacidae)

摘要

The SFI1/GNA fusion protein,comprising of snowdrop lectin(Galanthus nivalis agglutinin,GNA)fused to an insecticidal spider venom neurotoxin(Segestria florentina toxin 1,SFI1)was tested for toxicity against the rice brown planthopper Nilaparvata lugens(Stal)and the peach-potato aphid Myzus persicae(Sulzer)by incorporation into artificial diets.Significant effects on the mortality of N.lugens were observed,with 100% of the insects fed on the SFI1/GNA fusion protein diet dead by day 7.The survival of the aphid M.persicae was also reduced when fed on the SFI1/GNA fusion protein.After 14 days,only 49% of the aphids that were fed on the fusion protein were still alive compared with approximately 90% of the aphids fed on the control diet or on diet containing GNA only.The SFI1/GNA fusion protein also slowed the development of M.persicae,and the reproductive capacity of the aphids fed on the SFI1/GNA fusion protein was severely reduced.The ability of GNA to act as a carrier protein,and deliver the SFI1 neurotoxin to the haemolymph of N.lugens,following oral ingest ion,was investigated.The successful delivery of intact SFI1/GNA fusion protein to the haemolymph of these insects was shown by western blotting.Haemolymph taken from the insects that were fed on the fusion protein contained two GNA-immunoreactive proteins of molecular weights corresponding to GNA and to the SFI1/GNA fusion protein.? 2005 British Crown Copyright.Published for SCI by John Wiley & Sons,Ltd.