Gross morphology and localization of adenohypophyseal cells in camel (Camelus dromedarius) using a new combination of stains.

摘要

Thirty normal camels (Camelus dromedarius) were selected for gross morphological and modified staining of anterior pituitary. Camels were divided in three age groups viz 2-4, 5-10 and above 10 years. Pituitary weight, length, width and circumference were recorded before preservation and at midsegittal cutting. Pituitary weight increased significantly as these animals grew older. Male had heavier pituitary as compared to female. Higher pituitary weight was observed in old as compared to young camel. Sections (4 micro m) of camel pituitary gland were stained with "Phosphotungstic acid haematoxylin-Orange G-Acid fuchsin-Light green" combination of dyes. This combination of acidic and basic dyes showed affinity to their respective adenohypophyseal cells and proved a suitable combination for differentiation of adenohypophyseal cells and architectural pattern of pituitary gland. Use of Lugol's Iodine and sodium thiosulphate solution caused mercury fixation which ultimately enhanced the staining of camel adenohypophysis. The whole pituitary presented a brilliant appearance of clarity, enabling cell counts to be performed easily, purely with reference to the colors of adenohypophyseal cell types. This method can be applied for differential staining of adenohypophysis and with good cytology results to the hypophysis of many mammals. The method also provides a sharp contrast between cellular and connective tissue components. With this staining technique, the quantitative and qualitative characteristics of different adenohypophyseal cell types at various functional and hormonal stages, under certain physiological and pathological conditions can also be studied.