Pollen germination studies

摘要

Pollen germination tests have been reported to provide reasonable estimates of pollen fertility (Brewbaker and Kwack, 1963). Our objective was to test pollen germination of four PS (partial sterile) mutants and to make comparisons with the results ofpollen germination of fertile plants. The four PS mutants were recovered from a gene tagging study (Palmer et al., 1989). The mutants are male fertile, as judged by pollen staining and fluorescence (Pereira, 1994). PS-1 is inherited as a single recessive gene, and PS-2, PS-3, and PS-4 are maintained as heterozygotes and upon self-pollination segregate 1 fertile: 1 partial sterile plant. All four mutants are partial female sterile.