We are interested in using molecular plant breeding to improve the flooding tolerance of soybean. This technique requires the mapping of QTL and identification of DNA markers closely linked to the flood-tolerant trait. An efficient and reproducible screening technique is essential for QTL mapping. The majority of flooding research in the literature was conducted by complete submersion of seedlings 3- to 7-d-old in deoxygenated buffer for 1 to 4 days. At the end of the stress, the seedlings were allowed to grow and their viability determined. This type of screening technique can be done rather quickly and does not require a lot of space. The extrapolation of such screening results to field flooding tolerance has not been substantiated. In this study, we report the results of the field, greenhouse and laboratory screening tests of 21 soybean lines of different flood-tolerant levels in our collection.
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