Spectrophotometric determination of thiocyanate in human saliva employing micropumping multicommutation flow system

摘要

A procedure based on a multicommutated approach for the determination of thiocyanate (SCN~-) in human saliva is described. The method was based on the reaction of Fe(III) with SCN~-, producing a red complex that was monitored at 480nm. Under optimum experimental conditions, a linear response ranging from 0.5 to 10.0mmolL~(-1) SCN- (R=0.9991), a relative standard deviation of 1.0% (n=10) for a sample 5mmolL~(-1) SCN~(--), a detection limit (3σ criterion) of 50μmolL~(-1), a sampling rate of 60 determinations per hour, reagent consumption of 0.02mg Fe(III) per determination, and a waste generation of 1.6mL per determination were achieved.