Although many studies suggest that no-tillage (NT) increases soil organic carbon (SOC) within the soil profile relative to mouldboard ploughing, other studies indicate that no net change occurs. The latter studies suggest that NT only stratifies the SOC, where a near-surface increase in SOC is offset by a concomitant decrease in the subsurface. We examined the SOC distribution and stocks in a cool, humid Brookston clay (Typic Argiaquoll) soil under four soil management systems with a corn-soybean rotation. The objectives of this study were to compare the profile distribution and total amount of SOC under long-term (21 years) NT and mouldboard plough (MP) tillage with the changes that occur over 8 years when 13-years continuous NT is converted to MP, and when 13-years continuous MP is converted to NT. In the top 5cm of soil, the long-term NT management accumulated greater SOC compared with the long-term MP treatment. However, this near-surface increase was offset by lower SOC concentrations in the 10-20cm depth, resulting in similar total amounts of SOC stored in 0-20cm for both long-term NT and MP. The SOC stratification that existed after 13 years of NT management was eliminated with one mouldboard ploughing operation, however the total SOC content in the plough layer of the new-MP treatment remained relatively constant over the subsequent 8 years. Soil organic carbon stratification was evident in the new no-tillage treatment 3 years after the cessation of tillage. The continuous build-up of SOC in the surface of new-NT soils was associated with no change in the total amount of SOC in the plough layer relative to long-term NT. This implies that the diminution of SOC in the 10-20cm depth was at the same rate as the accumulation of SOC in the 0-5cm depth. Although there was no net effect of tillage on total carbon stocks in this fine-textured soil, SOC stratification required several years to build-up after adoption of NT, but only a single year to destroy under MP.
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