Nanocomposite Hydrogels as Platform for Cells Growth, Proliferation, and Chemotaxis

摘要

The challenge of mimicking the extracellular matrix with artificial scaffolds that are able to reduce immunoresponse is still unmet. Recent findings have shown that mesenchymal stem cells (MSC) infiltrating into the implanted scaffold have effects on the implant integration by improving the healing process. Toward this aim, a novel polyamidoamine-based nanocomposite hydrogel is synthesized, crosslinked with porous nanomaterials (i.e., mesoporous silica nanoparticles), able to release chemokine proteins. A comprehensive viscoelasticity study confirms that the hydrogel provides optimal structural support for MSC infiltration and proliferation. The efficiency of this hydrogel, containing the chemoattractant stromal cell-derived factor 1 alpha (SDF-1 alpha), in promoting MSC migration in vitro is demonstrated. Finally, subcutaneous implantation of SDF-1 alpha-releasing hydrogels in mice results in a modulation of the inflammatory reaction. Overall, the proposed SDF-1 alpha-nanocomposite hydrogel proves to have potential for applications in tissue engineering.