Protein Modification by Strain-Promoted Alkyne-Azide Cycloaddition

摘要

Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.