Characterization of the human myeloid cell nuclear differentiation antigen gene promoter

摘要

MNDA (myeloid cell nuclear differentiation antigen) is an interferon a regulated nuclear protein expressed only in cells of the human myelomonocylie lineage. To identify mechanisms responsible for this lineage-specific and interferon-regulated expression, the 5' flanking sequence of the gene has been characterized. Two inieri'eron-stimulated response elements (ISRE) flank a multiple transcription start site region identifying MNDA as a TATA-less interferon-regulated gene. Other DNA elements present include a cluster of Myb sites, several Ets, an Els related I'll.I site and an Spl site located within 600 bp of the transcription start sites. In addition, DNA methylation was revealed as one of the possible factors in establishing MNDA expression. The 5' flanking sequence has promoter activity which is elevated by interferon t. The findings indicate that MNDA expression is regulated by mechanisms similar to other myelomonocytic cell specific genes and genes up-reguluied by interferon a.