Studies on in vitro clonal propagation of Paulownia tomentosa STEUD. and evaluation of genetic fidelity through RAPD marker

摘要

Nodal explants were used for clonal propagation of Paulownia tomentosa by manipulating the cytokinin and auxin. Bud proliferation and multiple shoots were achieved from nodal explants derived from greenhouse grown plants of Paulownia tomentosa on Murashige and Skoog (MS) medium supplemented with 2.22-6.66 muM BA. Inclusion of NAA (0.53-1.34 muM) in the culture medium enhanced the rate of multiplication. The shoot length was attained 3-4 cm on MS medium supplemented with 4.44 muM BA + 0.53 muM NAA + 3% (w/v) sucrose within 4 weeks of culture. The rate of multiplication was maintain in subsequent subculture. Excised shoots were rooted on half-strength MS medium supplemented with 0.49 muM IBA after 2 weeks of culture. On an average of 81 plantlets were obtained from each nodal explant within 12-week of subculture. Rooted propagules were acclimatized and successfully transferred to greenhouse. The micropropagated plantlets appeared morphologically similar with the mother plants. No variation was detected among the micropropagated plants by the use of Randomly Amplified Polymorphic DNA (RAPD) markers.